Protein_Domain
MLS

Part:BBa_K1119000:Design

Designed by: Wai In Hui   Group: iGEM13_Hong_Kong_HKUST   (2013-09-11)

Mitochondrial Leader Sequence in RFC10 standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in RFC10 standard but cannot be fused directly to other protein domains due to limitations in RFC10. Users who obtained this part can obtain the part by PCR and fuse to other domains using Splicing by Overlapping PCR.

Source

The CDS of MLS was cloned out from pCMV/myc/mito (Invitrogen, Carlsbard, CA) using PCR with primers that includes prefix and suffix in RFC10 standard. The original source of this CDS of MLS sequence is from that of subunit VIII of human cytochrome c oxidase. (Invitrogen, Carlsbard, CA)

References

pcmv/myc/mito Invitrogen.(2012).pShooter™ Vector(pCMV/myc vectors).Retrieved from http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf
Dean, J., Tran , L., Liao, J., Dipple, K., Reue, K., Tontonoz, P., & Beaven, S. (2009). Resistance to diet-induced obesity in mice with synthetic glyoxylate shunt. Cell Metabolism, 9((6)), 525–536. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/19490907
French, A.P., S. Mills, R. Swarup, M.J. Bennett, and T.P. Pridmore. (2008). Colocalization of fluorescent markers in confocal microscope images of plant cells. Nature Protocol. 3:619–628. doi:10.1038/nprot.2008.31.